Immunoassay for sensitive and specific quantitative determination of human C-peptide in urine, serum or plasma.
Read the Directions for Use
Mercodia Ultrasensitive C-peptide ELISA provides a method for the quantitative determination of human C-peptide in serum, plasma or urine.
Format: 1 x 96 wells
Specificity: Little or no crossreactivity to insulin or proinsulin.
Samples: Serum, plasma, urine
Sample volume: 50 μL
Assay range: 5 - 280 pmol/L (0.015 - 0.846 ng/mL)
Detection limit: ≤ 2.5 pmol/L
Incubation (min): 60+60+30
The detection limit is ≤ 2.5 pmol/L (0.0076 μg/L) as determined by the methodology described in ISO11843- Part 4.
Serum: Recovery upon addition is 87 - 123% (mean 103%). Serum: Recovery upon dilution is 106 - 117% (mean 111%).
Each sample was analyzed in 4 replicates on 14 different occasions.
|Sample||Mean Value||Coefficient of variation|
|(pmol/L)||within assay %||between assay %||total assay %|
Serum, EDTA and heparin plasma can be used.
Grossly lipemic, icteric or hemolyzed samples do not interfere in the assay.
Mercodia Ultrasensitive C-peptide ELISA is a solid phase two- site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the C-peptide molecule. C-peptide in the sample reacts with anti-C-peptide antibodies bound to microtitration wells and peroxidase-conjugated anti- insulin antibodies in the solution.
Summary of protocol
The following cross-reactions have been tested:
|Insulin||< 0.0006 %|
|Proinsulin (Des 31-32)||3 %|
|Proinsulin (Split 32-33)||2 %|
|Proinsulin (Des 64-65)||74 %|
|Proinsulin (Split 65-66)||10 %|
Okada, M, Imai, T, Yaegaki, K (2014)
Regeneration of insulin-producing pancreatic cells using a volatile bioactive compound and human teeth
Espes, D, Lau, J, Carlsson, P (2013)
Increased circulating levels of betatrophin in individuals with long-standing type 1 diabetes
Oh, B, J, Oh, S (2014)
Co-culture with Mature Islet Cells Augments the Differentiation of Insulin-Producing Cells from Pluripotent Stem Cells