Immunoassay for quantitative determination of rat and mouse proinsulin in serum, plasma, cell culture media or tissue homogenate. No cross-reactivity to rat/mouse insulin or C-peptide. No cross-reactivity to human insulin, C-peptide or proinsulin.
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If controls are needed please contact Mercodia for further information.
Mercodia Rat/Mouse Proinsulin ELISA provides a method for the quantitative determination of proinsulin in rat and mouse serum, plasma, cell culture medium and cellular extracts.
Format: 1 x 96 wells/10 x 96 wells
Samples: Serum, plasma, cell culture media or tissue homogenate
Sample volume: 25 μL
Assay range: 3.5 - 200 pmol/L
Detection limit: 3 pmol/L
Incubation: (min) 120+15
The detection limit is 3 pmol/L as determined with the
methodology described in ISO11843-Part 4.
Recovery upon addition is 86 - 109 % (mean 98 %)
Recovery upon dilution is 85 - 107 % (mean 97 %)
Each sample was analyzed in 4 replicates on 22 different
|Sample||Mean Value||Coefficient of variation|
|(μg/L)||within assay %||between assay %||total assay %|
Serum, plasma, cell and tissue culture medium. Sample volume is 25 μL.
Mercodia Rat/Mouse Proinsulin ELISA is a solid phase two-
site enzyme immunoassay based on the sandwic technique,
in which two monoclonal antibodies are directed against
separate antigenic determinants on the proinsulin molecule.
Proinsulin in the sample reacts with anti-proinsulin antibodies
bound to microtitration wells and peroxidase-conjugated anti-
proinsulin antibodies in the solution.
Summary of protocol
The following cross-reactions have been tested:
|Human proinsulin||5 %|
n.d.= not detected
Ma, Z, Moruzzi, N, Catrina, S (2013)
Preconditioning with associated blocking of Ca2+ inflow alleviates hypoxia-induced damage to pancreatic beta-cells
Tersey, S, A, Nishiki, Y (2012)
Islet beta-cell endoplasmic reticulum stress precedes the onset of type 1 diabetes in the nonobese diabetic mouse model
Kautz, S, van Burck, L, Schuster, M (2012)
Early insulin therapy prevents beta cell loss in a mouse model for permanent neonatal diabetes (Munich Ins2(C95S))