The Mercodia Rat Insulin ELISA is based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin.
Please note that the 10 pack Mercodia Rat Insulin ELISA is delivered in bulk and is suitable if you run larger quantities of samples.
Read the Directions for Use
Mercodia Rat Insulin ELISA provides a method for the quantitative determination of insulin in rat serum or plasma.
Format: 10 x 96 wells
Samples: Serum, plasma
Sample volume: 10 μL
Assay range: 0.15-5.5 μg/L
Detection limit: ≤ 0.15 μg/L
Incubation: (min) 120+15
The detection limit is ≤ 0.15 μg/L as determined with the
methodology described in ISO11843-Part 4.
Recovery upon addition is 80 - 93 % (86 %).
Recovery upon dilution is 83 - 111 % (96 %).
Each sample was analyzed in 4 replicates on 16 different
|Sample||Mean Value||Coefficient of variation|
|(μg/L)||within assay %||between assay %||total assay %|
Serum, plasma. Sample volume 10μL (Alternative protocol using 5μL sample volume)
Grossly lipemic, icteric or haemolyzed samples do not interfere in the assay.
Mercodia Rat Insulin ELISA is a solid phase two-site enzyme
immunoassay based on the sandwich technique, in which two
monoclonal antibodies are directed against separate antigenic
determinants on the insulin molecule. Insulin in the sample reacts
with anti-insulin antibodies bound to microtitration wells and
peroxidase-conjugated anti-insulin antibodies in the solution.
Summary of protocol
The following cross-reactions have been tested:
|Human insulin||167 %|
|Human proinsulin||75 %|
|Human C-peptide||< 0.05 %|
|Insulin lispro||167 %|
|IGF-I||< 0.02 %|
|IGF-II||< 0.02 %|
|Rat C-peptide||< 0.001 %|
|Rat proinsulin||7 %|
|Porcine insulin||628 %|
|Ovine insulin||256 %|
|Bovine insulin||110 %|
Cross-reactivity in Rat and Mouse Insulin ELISA's.pdf
5 µL application Rat/Mouse assays.pdf
Detection of human and rat insulin in a sample.pdf
Detection of both porcine insulin and rat insulin in a sample.pdf
Instruction for test of microplate reader.pdf
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