The Rat Insulin ELISA is based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin.
Read the Directions for Use
*Read more about the handling of 5 μL sample volume in the Technical Note 34-0130
Mercodia Rat Insulin ELISA provides a method for the quantitative determination of insulin in rat serum, plasma or cell culture medium.
Format: 1 x 96 wells
Samples: Serum, plasma or cell culture medium
Sample volume: 10 μL (5 μL)
Assay range: 0.15-5.5 μg/L
Detection limit: ≤ 0.15 μg/L
Incubation: (min) 120+15
The detection limit is ≤ 0.15 μg/L as determined with the
methodology described in ISO11843-Part 4.
Recovery upon addition is 80 - 93 % (86 %).
Recovery upon dilution is 83 - 111 % (96 %).
Each sample was analyzed in 4 replicates on 16 different
|Sample||Mean Value||Coefficient of variation|
|(μg/L)||within assay %||between assay %||total assay %|
Mercodia Rat Insulin ELISA is a solid phase two-site enzyme immunoassay based on the sandwic technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the insulin molecule. Insulin in the sample reacts with anti-insulin antibodies bound to microtitration wells and peroxidase-conjugated anti-insulin antibodies in the solution.
Summary of protocol
The following cross-reactions have been found:
|Human insulin||167 %|
|Human proinsulin||75 %|
|Human C-peptide||< 0.05 %|
|Insulin lispro||167 %|
|IGF-I||< 0.02 %|
|IGF-II||< 0.02 %|
|Mouse Insulin||75 %|
|Rat C-peptide||< 0.001 %|
|Rat proinsulin||7 %|
|Porcine insulin||628 %|
|Ovine insulin||256 %|
|Bovine insulin||110 %|
Perry, R. J. et al. Mechanism for leptin’s acute insulin-independent effect to reverse diabetic ketoacidosis. J. Clin. Invest. 127, 657–669 (2017).
Fuhrmann, A, Lopes, P, Sereno, J (2014)
Molecular mechanisms underlying the effects of cyclosporin A and sirolimus on glucose and lipid metabolism in liver, skeletal muscle and adipose tissue in an in vivo rat model
Sun, T, Guo, J, Chen, H (2014)
Maternal caffeine exposure impairs insulin secretion by pancreatic beta-cells and increases the risk of type II diabetes mellitus in offspring
Luppi, P, Drain, P, (2014)
Autocrine C-peptide mechanism underlying INS1 beta cell adaptation to oxidative stress