Immunoassay for quantitative determination of rat C-peptide in serum, plasma or cell culture medium.
Related products: Sample buffer (for dilution of samples when needed) 10-1195-01
If controls are needed please contact Mercodia for further information
Read the Directions for Use
A high quality enzyme immunoassay for the quantification of
rat C-peptide in serum, plasma or cell culture medium.
Format: 1 x 96 wells
Specificity: No cross reactivity to rat insulin or rat proinsulin.
Samples: Serum, EDTA plasma and cell culture medium.
Sample volume: 10 μL
Assay range: 100 - 4000 pmol/L
Detection limit: ≤ 100 pmol/L (0.326 μg/L)
The detection limit is ≤ 100 pmol/L (0.326 μg/L) as determined with the methodology described in ISO11843-Part 4.
Recovery upon addition is 97 - 100 % (mean 98 %)
Recovery upon dilution is 91 - 105 % (mean 100 %)
Each sample was analyzed in 4 replicates on 24 different
|Sample||Mean Value||Coefficient of variation|
|(μg/L)||within assay %||between assay %||total assay %|
Serum, EDTA plasma and cell culture medium samples can be used. Grossly lipemic, icteric or haemolyzed samples do not interfere in the assay.
Mercodia Rat C-peptide ELISA is a solid phase two-site enzyme immunoassay based on the sandwic technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the C-peptide molecule. C-peptide in the sample reacts with anti-C-peptide antibodies bound to microtitration wells and peroxidase-conjugated anti- proinsulin antibodies in the solution.
Summary of protocol
The following cross-reactions have been tested:
|Rat insulin||< 0.01 %|
|Rat proinsulin||4.55 %|
|Human C-peptide||< 0.001 %|
Sacramento, J. F. et al. Functional abolition of carotid body activity restores insulin action and glucose homeostasis in rats: key roles for visceral adipose tissue and the liver. Diabetologia 60, 158–168 (2017).
Tsai, P, J, Wang, H (2012)
Transplantation of insulin-producing cells from umbilical cord mesenchymal stem cells for the treatment of streptozotocin-induced diabetic rats
Auberval, N, Dal, S, Bietiger, W (2014)
Metabolic and oxidative stress markers in Wistar rats after 2 months on a high-fat diet
Gu, L, H, Zhang, T (2014)
Immunogenicity of allogeneic mesenchymal stem cells transplanted via different routes in diabetic rats