The Mercodia Porcine Insulin ELISA is a species-optimized, high quality enzyme immunoassay for the quantification of porcine insulin in serum or plasma.
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Available control set: Mercodia Animal Insulin Control (Low, Medium, High), 10-1221-01
Mercodia Porcine Insulin ELISA provides a method for the quantitative determination of insulin in porcine serum and plasma.
Format: 1 x 96 wells
Specificity: No cross-reactivity to C-peptide or proinsulin
Samples: Serum, plasma
Sample volume: 25 μL
Assay range: 2.3 - 173 mU/L
Detection limit: ≤ 1.15 mU/L
Incubation (min): 120+15
The detection limit is ≤ 1.15 mU/L as determined with the
methodology described in ISO11843-Part 4.
Recovery upon addition is 100 - 111 % (105 %).
Recovery upon dilution is 90 - 112 % (97 %).
Each sample was analyzed in 4 replicates on 13 different
|Sample||Mean Value||Coefficient of variation|
|(mU/L)||within assay %||between assay %||total assay %|
Serum or plasma, sample volume: 25μL.
Grossly lipemic, icteric or haemolyzed samples do not interfere in the assay.
Mercodia Porcine Insulin ELISA is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the insulin molecule. Insulin in the samplereacts with anti-insulin antibodies bound to microtitration wells and peroxidase-conjugated anti-insulin antibodies in the solution.
Summary of protocol
The following cross-reactions have been tested:
|Porcine C-peptide||< 0.001 %|
|Porcine proinsulin||< 0.2 %|
|Equine insulin||100 %|
|Canine insulin||100 %|
|Human insulin||108 %|
|Human C-peptide||< 0.01 %|
|Human proinsulin||< 0.1 %|
n.d.= not detected
Kemter, E., Cohrs, C.M., Schäfer, M. et al. Diabetologia (2017) 60: 1152. https://doi.org/10.1007/s00125-017-4250-2
Lindqvist, A, Spegel, P, Ekelund, M (2014)
Gastric bypass improves ss-cell function and increases beta-cell mass in a porcine model
Kelly, A, C, Steyn, L (2014)
Function and expression of sulfonylurea, adrenergic, and glucagon-like peptide 1 receptors in isolated porcine islets
SoRelle, J, A, Kanak, M (2014)
Comparison of surface modification chemistries in mouse, porcine, and human islets