The Mercodia Ovine Insulin ELISA is a species-optimized, high quality enzyme immunoassay for the quantification of ovine insulin in serum or plasma.
Read the Directions for Use
Available Control sets:
Mercodia Insulin Control Animal Low, Medium & High 10-1221-01
Mercodia Ovine Insulin ELISA provides a method for the quantitative determination of insulin in ovine serum and plasma.
Format: 1 x 96 wells
Specificity: No crossreactivity to C-peptide or proinsulin.
Samples: Serum, plasma
Sample volume: 25 μL
Assay range: 0.05 - 3.0 μg/L
Detection limit: ≤ 0.025 μg/L
Incubation (min): 120+15
The detection limit is ≤ 0.025 μg/L as determined with the
methodology described in ISO11843-Part 4.
Recovery upon addition is 94 - 114 % (103 %).
Recovery upon dilution is 68 - 122 % (89 %).
Each sample was analyzed in 4 replicates on 19 different
|Sample||Mean Value||Coefficient of variation|
|(μg/L)||within assay %||between assay %||total assay %|
Serum or plasma. Sample volume 25μL.
Grossly lipemic, icteric or haemolyzed samples do not interfere
in the assay.
Mercodia Ovine Insulin ELISA is a solid phase two-site enzyme
immunoassay based on the sandwich technique, in which two
monoclonal antibodies are directed against separate antigenic
determinants on the insulin molecule. Insulin in the sample
reacts with anti-insulin antibodies bound to microtitration
wells and peroxidase-conjugated anti-insulin antibodies in the
Summary of protocol
The following cross-reactions have been tested:
n.d.= not detected
Mahjoubi, E, Amanlou, H, Mirzaei-Alamouti, H (2014)
The effect of cyclical and mild heat stress on productivity and metabolism in Afshari lambs
Serment, A, Schmidely, P, Giger-Reverdin, S (2011)
Effects of the percentage of concentrate on rumen fermentation, nutrient digestibility, plasma metabolites, and milk composition in mid-lactation goats
Avondo, M, Pennisi, P, Lanza, M (2014)
Effect of the αs1-casein genotype and its interaction with diet degradability on milk production, milk quality, metabolic and endocrinal response of Girgentana goats