The Mercodia Mouse Insulin ELISA is an easy-to-use assay based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin.
Please note that the 10 pack Mercodia Mouse Insulin ELISA is delivered in bulk and is suitable if you run larger quantities of samples.
Read the Directions for Use
*Read more about the handling of 5 μL sample volume in the alternative protocol.
Mercodia Mouse Insulin ELISA provides a method for the quantitative determination of insulin in mouse serum or plasma.
Format: 10 x 96 wells
Samples: Serum, plasma
Sample volume: 10 μL
Assay range: 0.2 - 6.5 μg/L
Detection limit: ≤ 0.2 μg/L
Incubation: (min) 120+15
The detection limit is ≤ 0.2 μg/L as determined with the
methodology described in ISO11843-Part 4.
Recovery upon addition is 100 - 130 % (111 %).
Recovery upon dilution is 109 - 149 % (130 %).
Each sample was analyzed in 4 replicates on 16 different
|Sample||Mean Value||Coefficient of variation|
|(μg/L)||within assay %||between assay %||total assay %|
Serum, plasma, cell culture medium. Sample volume 10μL (5μL).
Grossly lipemic, icteric or haemolyzed samples do not interfere
in the assay.
Mercodia Mouse Insulin ELISA is a solid phase two-site enzyme
immunoassay based on the sandwich technique, in which two
monoclonal antibodies are directed against separate antigenic
determinants on the insulin molecule. Insulin in the sample reacts
with anti-insulin antibodies bound to microtitration wells and
peroxidase-conjugated anti-insulin antibodies in the solution
Summary of protocol
The following cross-reactions have been tested:
|Mouse C-peptide I||n.d.|
|Mouse C-peptide II||n.d.|
|Rat C-peptide I||n.d.|
|Rat C-peptide II||n.d.|
|Rat insulin||146 %|
|Mouse proinsulin I||43 %|
|Mouse proinsulin II||60 %|
|Rat proinsulin I||14 %|
|Rat proinsulin II||60 %|
|Porcine insulin||628 %|
|Ovine insulin||256 %|
|Bovine insulin||110 %|
|Human insulin||195 %|
|Human proinsulin||82 %|
Stenkula, K, G, Lindahl, M (2014)
Single injections of apoA-I acutely improve in vivo glucose tolerance in insulin-resistant mice
Oh, B, J, Oh, S (2014)
Co-culture with Mature Islet Cells Augments the Differentiation of Insulin-Producing Cells from Pluripotent Stem Cells
Mastrocola, R, Collino, M, Nigro, D (2015)
Accumulation of Advanced Glycation End-Products and Activation of the SCAP/SREBP Lipogenetic Pathway Occur in Diet-Induced Obese Mouse Skeletal Muscle
Rachel J. Perry, Liang Peng, Abudukadier Abulizi
Mechanism for leptin’s acute insulin-independent effect to reverse diabetic ketoacidosis