The Mercodia Mouse Insulin ELISA is an easy-to-use assay based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin.
Read the Directions for Use
Mercodia Mouse Insulin ELISA provides a method for the quantitative determination of insulin in mouse serum, plasma or cell culture medium.
Format: 1 x 96 wells
Samples: serum, plasma or cell culture medium
Sample volume: 10 μL (5 μL, see TN34-0130)
Assay range: 0.2 - 6.5 μg/L
Detection limit: ≤ 0.2 μg/L
Incubation: (min) 120+15
The detection limit is ≤ 0.2 μg/L as determined with the
methodology described in ISO11843-Part 4.
Recovery upon addition is 100 - 130 % (111 %).
Recovery upon dilution is 109 - 149 % (130 %).
Each sample was analyzed in 4 replicates on 16 different
|Sample||Mean Value||Coefficient of variation|
|(μg/L)||within assay %||between assay %||total assay %|
Mercodia Mouse Insulin ELISA is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the insulin molecule. Insulin in the sample reacts with anti-insulin antibodies bound to microtitration wells and peroxidase-conjugated anti-insulin antibodies in the solution.
Summary of protocol
The following cross-reactions have been found:
|Mouse C-peptide I||n.d.|
|Mouse C-peptide II||n.d.|
|Rat C-peptide I||n.d.|
|Rat C-peptide II||n.d.|
|Rat insulin||146 %|
|Mouse proinsulin I||43 %|
|Mouse proinsulin II||60 %|
|Rat proinsulin I||14 %|
|Rat proinsulin II||60 %|
|Porcine insulin||628 %|
|Ovine insulin||256 %|
|Bovine insulin||110 %|
|Human insulin||195 %|
|Human proinsulin||82 %|
Stenkula, K, G, Lindahl, M (2014)
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Oh, B, J, Oh, S (2014)
Co-culture with Mature Islet Cells Augments the Differentiation of Insulin-Producing Cells from Pluripotent Stem Cells
Mastrocola, R, Collino, M, Nigro, D (2015)
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