A high quality enzyme immunoassay for specific quantification of human insulin in serum or plasma.
The Mercodia insulin assays have little or no cross-reactivity to insulin analogs, rat or mouse insulin, which enables distinguishing endogenous human insulin, both in patients undergoing treatment with insulin analogs, and in rat or mouse models.
Read the Directions for Use
Related products: Sample buffer 10-1195-01 (for dilution of samples when needed)
Also available as 10 pack 10-1113-10
Mercodia Insulin ELISA provides a method for the quantitative determination of human insulin in serum or plasma.
Format: 1 x 96 wells
Specificity: No crossreactivity to C-peptide or proinsulin.
Samples: Serum, plasma
Sample volume: 25 μL
Assay range: 3 - 200 mU/L
Detection limit: ≤ 1 mU/L (0.043 μg/L)
Incubation (min): 60+15
The detection limit is ≤ 1 mU/L (0.043 μg/L) calculated as two standard deviations above the Calibrator 0.
1μg/L = 23 mU/L; 1 mU/L = 6.0 pmol/L
Recovery upon addition is 94 - 113 % (mean 104 %).
Each sample was analyzed in 6 replicates on 6 different occasions
|Sample||Mean Value||Coefficient of variation|
|(μg/L)||within assay %||between assay %||total assay %|
Serum, EDTA and heparin plasma can be used.
Grossly lipemic, icteric or hemolyzed samples do not interfere in the assay. However, hemolysis in serum and plasma samples may result in a degradation of insulin which could give falsely low values and contributes to higher inter-assay variation. The degradation is dependent on time, temperature and the hemoglobin concentration. Keep hemolyzed samples cold or on ice to prevent the insulin degradation.
Mercodia Insulin ELISA is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the insulin molecule. Insulin in the sample reacts with anti-insulin antibodies bound to microtitration wells and peroxidase-conjugated anti-insulin antibodies in the solution.
Summary of protocol
The following cross-reactions have been tested:
|Proinsulin des (31-32)||<0.5 %|
|Proinsulin split (32-33)||<0.5 %|
|Proinsulin des (64-65)||98 %|
|Proinsulin split (65-66)||56 %|
|Insulin aspart||4 %|
|Insulin glargin||24 %|
|Rat insulin||0.7 %|
|Mouse insulin||0.3 %|
|Porcine insulin||374 %|
|Ovine insulin||48 %|
|Bovine insulin||31 %|
Cross-reactivity human insulin analogs.pdf
Detection of Human and Mouse insulin in a sample
Detection of Human and Rat insulin in a sample
Analysis of insulin, C-peptide or proinsulin from acid ethanol extractions from islets, cells or tissue.pdf
Comparison of Insulin ELISA and Iso-Insulin ELISA.pdf
Insulin Pre-Transplantation application.pdf
Instruction manual wash.pdf
Instruction for test of microplate reader.pdf
MSDS Insulin ELISA
Iso-Insulin ELISA and Glargine handout.pdf
Poster Risk assessment for diabetes.pdf
Dhawan, S., Dirice, E., Kulkarni, R. N. & Bhushan, A. Inhibition of TGF-beta signaling promotes human pancreatic beta cell replication. Diabetes 65, db15-1331- (2016)
Walhin, J, P, Richardson, J (2013)
Exercise counteracts the effects of short-term overfeeding and reduced physical activity independent of energy imbalance in healthy young men
Allen, E, Gray, P, Kollias-Pearson, A (2014)
The effect of short-duration sprint interval exercise on plasma postprandial triacylglycerol levels in young men
Poquette, N, M, Gu, X (2014)
Grain sorghum muffin reduces glucose and insulin responses in men