The Mercodia Glucagon ELISA is a CE/IVD-marked, highly specific and sensitive immunoassay for measuring glucagon in plasma and serum.
The highly specific monoclonal antibodies used in this assay enables accurate determination of glucagon without significant cross-reactivity to other circulating pro-glucagon derived peptides, like for instance glicentin. Circulating glicentin often cause cross-reactivity problems because of its shared sequence with glucagon.
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Read our White Paper on the challenges of measuring glucagon.
Read the White Paper : Glucagon metabolic Regulator
Under certain conditions like after bariatric surgery, kidney disease and exogenous administration of glucagon, pro-glucagon deriviatives can be elevetade and, hence, increased assay specificity is needed. The alternative sequential assay protocol, TN34-0158, is recommended for these patient subgroups. TN34-0158.
Validated in human, rat and mouse. For other species, please visit the Mercodia Glucagon ELISA -10μL product page.
Mercodia Glucagon ELISA is an assay intended to measure the pancreatic hormone glucagon in plasma and serum. Glucagon measurements are used in the diagnosis and treatment of patients with various disorders of carbohydrate metabolism, including diabetes mellitus, hypoglycemia, and hyperglycemia.
Format: 1 x 96 wells
Samples: Serum, EDTA plasma and cell culture medium.
Sample volume: 25 μL
Assay range: 1.5-120 pmol/L (5-414 pg/mL)
Detection limit: ≤ 1 pmol/L (3.49 pg/mL)
Incubation: 18-22 + 15 h (overnight)
The detection limit is ≤ 1 pmol/L (3.49 pg/mL) as determined with the methodology described in ISO11843- Part 4.
Recovery upon addition 96–101% (Mean 98%)
Recovery upon dilution 81–96% (Mean 86%)
Each sample was analyzed in 4 replicates on 39 different occasions
|Sample||Mean Value||Coefficient of variation|
|(pmol/L)||within assay %||between assay %||total assay %|
Serum, EDTA plasma and cell culture medium samples can be used.
Grossly lipemic, icteric or haemolyzed samples do not interfere in the assay.
For studies in which very low levels of glucagon need to be detected, it may be beneficial to use BD (Becton Dickinson) P800 tubes for sample collection, since this will prevent the degradation of glucagon. If possible, avoid storing samples at room temperature or 2-8°C for long periods of time. Avoid freeze-thaw cycles.
Mercodia Glucagon ELISA is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the glucagon molecule. Glucagon in the sam- ple reacts with anti-glucagon antibodies bound to microtitration wells and peroxidase-conjugated anti-glucagon antibodies in the solution.
Summary of protocols
The following cross-reactions have been tested:
|GLP-1 (1-36 amide)||<0.30 %|
|GLP-1 (9-36 amide)||<0.30 %|
|GLP-1 (7-37)||<0.30 %|
Glucagon ELISA MSDS
Altered Plasma Levels of Glucagon, GLP-1 and Glicentin During OGTT in Adolescents With Obesity and Type 2 Diabetes.
Read the abstract
Hall, M, J, Adin, C (2014)
Pharmacokinetics and pharmacodynamics of the glucagon-like peptide-1 analog liraglutide in healthy cats
Wewer Albrechtsen, N, J, Hartmann, B (2014)
Hyperglucagonaemia analysed by glucagon sandwich ELISA: nonspecific interference or truly elevated levels?
Chow, S, Z, Speck, M (2014)
Gp130 receptor signaling mediates alpha cell dysfunction in a rodent model of type 2 diabetes
Malmgren, S, Ahren, B, (2015)
DPP-4 inhibition contributes to the prevention of hypoglycaemia through a GIP-glucagon counterregulatory axis in mice
Damond, N, Thorel, F, (2016)
Blockade Of Glucagon Signaling Prevents Or Reverses Diabetes Onset Only If Residual β-Cells Persist
Daniel Oropeza, Nathalie Jouvet, (2015)
Phenotypic Characterization of MIP-CreERT1Lphi Mice With Transgene-Driven Islet Expression of Human Growth Hormone