A high quality enzyme immunoassay for the quantification of human glicentin in serum, EDTA plasma and cell culture media.
Glicentin is a pro-glucagon derived gut peptide that is released in the gut in response to feed intake. It is present in high levels in the circulation after stimulation and is often a problem when measuring glucagon because of it's shared sequence.
Read the Directions for Use
Mercodia Glicentin ELISA provides a method for the quantitative determination of human glicentin in serum, EDTA plasma and cell culture medium.
Format: 1 x 96 wells
Specificity: No crossreactivity to proglucagon derived gut peptides
Samples: Serum or EDTA plasma
Sample volume: 25 μL
Assay range: 3 - 300 pmol/L (24-2400 pg/mL)
Detection limit: 3 pmol/L (24 pg/mL)
Incubation (min): 120+15
The detection limit is 3 pmol/L (24 pg/mL), as determined with the methodology described in ISO11843- Part 4.
Recovery upon addition 94–101% (mean 98%)
Recovery upon dilution 93–112% (mean 99%)
Each sample was analyzed in 2 replicates on 20 different occasions.
|Sample||Mean Value||Coefficient of variation|
|(pmol/L)||within assay %||between assay %||total assay %|
Serum, EDTA plasma and cell culture media samples can be used.
Grossly lipemic, icteric or haemolyzed samples do not interfere in the assay.
Mercodia Glicentin ELISA is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the glicentin molecule. Glicentin in the sample reacts with anti-glicentin antibodies bound to microtitration wells and peroxidase-conjugated anti-glicentin antibodies in the solution.
Summary of protocol
The following cross-reactions have been tested:
|Rat glicentin||0.9 %|