The Mercodia Feline Insulin ELISA is a species-optimized, high quality enzyme immunoassay for the quantification of feline insulin in serum or plasma.
Read the Directions for Use
Available Control sets:
Mercodia Insulin Control Animal Low, Medium & High 10-1221-01
The Mercodia Feline Insulin ELISA provides a method for the direct quantitative determination of insulin levels in feline serum or plasma samples.
Format: 1 x 96 wells
Specificity: No crossreactivity to C-peptide or proinsulin.
Samples: Serum, plasma
Sample volume: 10 μL
Assay range: 10 - 700 μg/L
Detection limit: ≤ 9.2 ng/L
Incubation (min): 120+15
The detection limit is ≤ 9.2 ng/L as determined with the
methodology described in ISO11843-Part 4.
Recovery upon addition is 84 - 101 % (107 %).
Recovery upon dilution is 91 - 128 % (95 %).
Each sample was analyzed in 4 replicates on 16 different occasions. The analysis were done in 2 laboratories with 2 kit lots and by 4 technicians.
Serum and plasma. Samnple volume 10μL.
Grossly lipemic, icteric or haemolyzed samples do not interfere
in the assay.
Mercodia Feline Insulin ELISA is a solid phase two-site enzyme
immunoassay based on the sandwich technique, in which two
monoclonal antibodies are directed against separate antigenic
determinants on the insulin molecule. Insulin in the sample
reacts with anti-insulin antibodies bound to microtitration
wells and peroxidase-conjugated anti-insulin antibodies in the
Summary of protocol
The following cross-reactions have been tested:
|NovoRapid® (Insulin aspart)||5.9 %|
|Levemir® (Insulin detemir)||< 0.008 %|
|Lantus® (Insulin glargin)||8.4 %|
|Humalog® (Insulin lispro)||n.d.|
|Apidra® (Insulin glulisine)||n.d.|
|Vetsulin®, Caninsulin®||57.4 %|
Deng, P, Ridge, T, K (2013)
Effects of dietary macronutrient composition and feeding frequency on fasting and postprandial hormone response in domestic cats
Strage, E, M, Holst, B (2012)
Validation of an enzyme-linked immunosorbent assay for measurement of feline serum insulin
Hall, M, J, Adin, C (2014)
Pharmacokinetics and pharmacodynamics of the glucagon-like peptide-1 analog liraglutide in healthy cats