The Mercodia Equine Insulin ELISA is a species-optimized, high quality enzyme immunoassay for the quantification of equine insulin in serum or plasma.
Read the Directions for Use
Available control set: Mercodia Animal Insulin Control (Low, Medium, High), 10-1221-01
Mercodia Equine Insulin ELISA provides a method for the quantitative determination of insulin in equine serum and plasma.
Format:1 x 96 wells
Specificity: No crossreactivity to C-peptide or proinsulin
Samples: Serum, plasma
Sample volume: 25 μL
Assay range: 2.3 - 173 mU/L
Detection limit: ≤ 1.15 mU/L
Incubation (min): 120+15
The detection limit is ≤ 1.15 mU/L as determined with the
methodology described in ISO11843-Part 4.
Recovery upon addition is 102 - 125 % (115 %).
Recovery upon dilution is 78 - 93 % (86 %).
Each sample was analyzed in 4 replicates on 28 different
|Sample||Mean Value||Coefficient of variation|
|(mU/L)||within assay %||between assay %||total assay %|
Serum, EDTA, heparin and citrate plasma can be used. Sample volume: 25μL.
Grossly lipemic, icteric or hemolyzed samples do not interfere in the assay.
Mercodia Equine Insulin ELISA is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the insulin molecule. Insulin in the sample reacts with anti-insulin antibodies bound to microtitration wells and peroxidase-conjugated anti-insulin antibodies in the solution.
Summary of protocol
The following cross-reactions have been found:
|Porcine Insulin||100 %|
|Porcine C-peptide||< 0.001 %|
|Porcine Proinsulin||< 0.2 %|
|Human Insulin||108 %|
|Human C-peptide||< 0.01 %|
|Human Proinsulin||< 0.1 %|
Brojer, J, Lindase, S, Hedenskog, J (2013)
Repeatability of the Combined Glucose-Insulin Tolerance Test and the Effect of a Stressor before Testing in Horses of 2 Breeds
Brunner, J, Liesegang, A, Weiss, S (2014)
Feeding practice and influence on selected blood parameters in show jumping horses competing in Switzerland
Brojer, J. Lindase, S. Hedenskog
Repeatability of the Combined Glucose-Insulin Tolerance Test and the Effect of a Stressor before Testing in Horses of 2 Breeds (2013)
T. Warnken, K. Huber and K. Feige (2016)
Comparison of three different methods for the quantification of equine insulin