The Mercodia Canine Insulin ELISA is a species-optimized, high quality enzyme immunoassay for the quantification of canine insulin in serum or plasma.
Read the Directions for Use
Available control set: Mercodia Animal Insulin Control (Low, Medium, High), 10-1221-01
Mercodia Canine Insulin ELISA provides a method for the quantitative determination of insulin in canine serum and plasma.
Format: 1 x 96 wells
Specificity: No crossreactivity to C-peptide or proinsulin.
Samples: Serum, plasma
Sample volume: 25 μL
Assay range: 2.3 - 173 mU/L
Detection limit: ≤ 1.15 mU/L
Incubation: (min) 120+15
The detection limit is ≤ 1.15 mU/L as determined with the
methodology described in ISO11843-Part 4.
Recovery upon addition is 103 - 124 % (111 %).
Recovery upon dilution is 75 - 102 % (90 %).
Each sample was analyzed in 4 replicates on 13 different
|Sample||Mean Value||Coefficient of variation|
|(mU/L)||within assay %||between assay %||total assay %|
Serum and plasma. Sample volume: 25μL.
Grossly lipemic, icteric or hemolyzed samples do not interfere in the assay.
Mercodia Canine Insulin ELISA is a solid phase two-site enzyme
immunoassay based on the sandwich technique, in which two
monoclonal antibodies are directed against separate antigenic
determinants on the insulin molecule. Insulin in the sample
reacts with anti-insulin antibodies bound to microtitration
wells and peroxidase-conjugated anti-insulin antibodies in the
Summary of protocol
The following cross-reactions have been found:
|Porcine Insulin||100 %|
|Porcine C-peptide||< 0.001 %|
|Porcine proinsulin||< 0.2 %|
|Equine insulin||100 %|
|Human insulin||108 %|
|Human C-peptide||< 0.01 %|
|Human proinsulin||< 0.1 %|
Tvarijonaviciute, A, Carrillo-Sanchez, J, D (2013)
Effect of estradiol and progesterone on metabolic biomarkers in healthy bitches
Seita, M, Noguchi, H, Kubota, Y (2013)
Development of Canine Models of Type 1 Diabetes With Partial Pancreatectomy and the Administration of Streptozotocin
Muller, L, Kollar, E, Balogh, L (2014)
Body fat distribution and metabolic consequences - Examination opportunities in dogs