A high quality immunoassay for specific quantification of human C-peptide in serum, plasma, cell culture media or urine. Due to the high specificity of the assays, Mercodia C-peptide ELISA's can also be used for determining human C-peptide in rat or mouse models.
Read the Directions for Use
10-1134-01 Control, Diabetes antigen - Human Low and High (unassayed - US only)
10-1164-01 Control, Diabetes antigen - Human Low and High
Mercodia C-peptide ELISA provides a method for the quantitative determination of human C-peptide in serum, plasma or urine.
Format: 1 x 96 wells
Specificity: Little or no crossreactivity to insulin or proinsulin.
Samples: serum, plasma, urine
Sample volume: 25 μL
Assay range: 100-4000 pmol/L (0.3-12.0 ng/mL)
Detection limit: ≤ 25 pmol/L (0.076 μg/L)
Incubation (min): 60+60+15
The detection limit is ≤ 25 pmol/L (0.076 μg/L) calculated as two standard deviations above the Calibrator 0.
Serum: Recovery upon addition is 93 - 113 % (mean 104 %). Urine: Recovery upon addition is 94 - 107 % (mean 99 %).
Each sample was analyzed in 4 replicates on 7 different occasions.
|Sample||Mean Value||Coefficient of variation|
|(μg/L)||within assay %||between assay %||total assay %|
Serum, EDTA and heparin plasma or urine can be used.
Grossly lipemic, icteric or haemolyzed samples do not interfere in the assay.
Mercodia C-peptide ELISA is a solid phase two-site enzyme
immunoassay based on the sandwich technique, in which
two monoclonal antibodies are directed against separate antigenic determinants on the C-peptide molecule. C-peptide in the sample reacts with anti-C-peptide antibodies bound to microtitration wells and peroxidase-conjugated anti-insulin antibodies in the solution.
Summary of protocol
The following cross-reactions have been tested:
|Insulin||< 0.0006 %|
|Proinsulin (Des 31-32)||3 %|
|Proinsulin (Split 32-33)||2 %|
|Proinsulin (Des 64-65)||74 %|
|Proinsulin (Split 65-66)||10 %|
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