The Mercodia Bovine Insulin ELISA is a high quality, species-optimized enzyme immunoassay for the quantification of bovine insulin in serum or plasma.
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Available Control sets: Mercodia Insulin Control Animal
Mercodia Bovine Insulin ELISA provides a method for the quantitative determination of insulin in bovine serum and plasma.
Format: 1 x 96 wells
Specificity: No crossreactivity to C-peptide or proinsulin.
Samples: Serum, plasma
Sample volume: 25 μL
Assay range: 0.05 - 3.0 μg/L
Detection limit: ≤ 0.025 μg/L
Incubation: (min) 120+15
The detection limit is ≤ 0.025 μg/L as determined with the methodology described in ISO11843-Part 4.
Recovery upon addition is 86 - 107 % (96 %).
Recovery upon dilution is 91 - 131 % (107 %).
Each sample was analyzed in 4 replicates on 20 different occasions.
|Sample||Mean Value||Coefficient of variation|
|(μg/L)||within assay %||between assay %||total assay %|
Serum or plasma. Sample volume: 25μL.
Grossly lipemic, icteric or haemolyzed samples do not interfere in the assay.
Mercodia Bovine Insulin ELISA is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the insulin molecule. Insulin in the sample reacts with anti-insulin antibodies bound to microtitration wells and peroxidase-conjugated anti-insulin antibodies in the solution.
Summary of protocol
The following cross-reactions have been tested:
|Levemir||< 0.0000004 %|
|Humalog||< 0.000001 %|
Ribeiro, E, S, Bruno, R (2014)
Low doses of bovine somatotropin enhance conceptus development and fertility in lactating dairy cows
Martinez, N, Sinedino, L, D (2014)
Effect of induced subclinical hypocalcemia on physiological responses and neutrophil function in dairy cows
Ollikainen, P, Muuronen, K, (2013)
Determination of insulin-like growth factor-1 and bovine insulin in raw milk and its casein and whey fractions after microfiltration and ultrafiltration