A high quality enzyme immunoassay for the quantification of human adiponectin in serum or plasma.
Read the Directions for Use
Available Controls: 10-1241-01 Control, Obesity A, B, C / Human
Mercodia Adiponectin ELISA provides a method for the quantitative determination of human adiponectin in serum or plasma.
Format: 1 x 96 wells
Samples: serum, plasma
Sample volume: 25 μL
Assay range: 5 - 300 ng/mL
Detection limit: ≤ 1.25 ng/mL
Incubation (min): 60+60+15
The detection limit is ≤ 1.25 ng/mL as determined with the methodology described in ISO11843-Part 4.
Recovery upon addition is 92 - 109 % (101 %).
Recovery upon dilution is 89 - 111 % (98 %).
Each sample was analyzed in 4 replicates on 39 different occasions.
|Sample||Mean Value||Coefficient of variation|
|(μg/L)||within assay %||between assay %||total assay %|
Serum, EDTA , citrate and heparin plasma can be used. Sample volume: 25 μL.
Grossly lipemic, icteric or haemolyzed samples do not interfere in the assay.
Mercodia Adiponectin ELISA is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the adiponectin molecule. Adiponectin in the sample reacts with anti-adiponectin antibodies bound to microtitration wells and peroxidase-conjugated anti-adiponectin antibodies in the solution.
Summary of protocol
|C1q||< 0.007 %|
N.D.= Non detectable
Instruction manual wash.pdf
Expected sample values for Mercodia Adiponectin ELISA
Validation of Mercodia Adiponectin ELISA using NHP samples
Instruction for test of microplate reader.pdf
Canivell, S, Ruano, E, G (2013)
Gastric Inhibitory Polypeptide Receptor Methylation in Newly Diagnosed, Drug-Naive Patients with Type 2 Diabetes: A Case-Control Study
Song, J, Kwon, N, Lee, M (2014)
Association of serum phospholipid PUFAs with cardiometabolic risk: Beneficial effect of DHA on the suppression of vascular proliferation/inflammation
Yoo, J, K, Hwang, M (2015)
Higher levels of adiponectin in vascular endothelial cells are associated with greater brachial artery flow-mediated dilation in older adults