Immunoassay for quantitative determination of human lipoprotein(a) in serum or plasma.
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Mercodia Lp(a) ELISA provides a method for the quantitative determination of human Lp(a) in serum or plasma.
Format: 1 x 96 wells
Samples: Serum, plasma
Sample volume: 25 μL
Assay range: 0.3 - 5.0 U/L
Detection limit: 0.05 U/L
Incubation: (min) 60+15
The detection limit is 0.05 U/L calculated as three standard deviations above the Calibrator 0.
Recovery upon addition is 96 - 111 % (102 %).
Samples pretreated and diluted 1/202 on one occasion and stored at –20°C until the assays were performed. Each sample was analysed in 4 replicates on 9 different occasions
|Sample||Mean Value||Coefficient of variation|
|(U/L)||within assay %||between assay %||total assay %|
Samples pretreated and diluted 1/202 on each test occasion.
Each sample was analysed in 5 replicates on 5 different occasions.
Serum, EDTA and heparin plasma can be used. Grossly lipemic, icteric or haemolyzed samples do not interfere in the assay.
Mercodia Lp(a) ELISA is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the apolipoprotein(a) molecule. Apolipoprotein(a) in the sample reacts with anti-apolipoprotein(a) antibodies bound to microtitration wells and peroxidase-conjugated anti-
apolipoprotein(a) antibodies in the solution.
Summary of protocol
The following cross-reactions have been tested:
n.d.= not detected
Clifton-Bligh, P, B, Nery, M (2014)
Red clover isoflavones enriched with formononetin lower serum LDL cholesterol-a randomized, double-blind, placebo-controlled study
Saeedi, R, Li, M, Allard, M (2014)
Marked effects of extreme levels of lipoprotein(a) on estimation of low-density lipoprotein cholesterol
Enkhmaa, B, Abbuthalha, A, Anuurad, E (2015)
Rhesus monkey (Macaca mulatta) lipoprotein(a) and apolipoprotein(a): high frequency of small size apolipoprotein(a) isoforms