A high quality enzyme immunoassay for the quantification of free human leptin in serum or plasma.
Read the Directions for Use
Mercodia Leptin ELISA provides a method for the quantitative determination of human leptin in serum and plasma.
Format: 1 x 96 wells
Samples: Serum, plasma
Sample volume: 25 μL
Assay range: 0.10 - 5.0 ng/mL
Detection limit: ≤ 0.024 ng/mL
Incubation (min): 120+15
The detection limit is ≤ 0.024 ng/mL as determined with the
methodology described in ISO11843-Part 4.
Recovery upon addition is 91 - 109 % (96 %).
Recovery upon dilution is 92 - 114 % (102 %).
Each sample was analyzed in 4 replicates on 24 different
|Sample||Mean Value||Coefficient of variation|
|(μg/L)||within assay %||between assay %||total assay %|
Serum, EDTA- , citrate- and heparin plasma can be used.
Grossly lipemic, icteric or haemolyzed samples do not interfere
in the assay.
Mercodia Leptin ELISA is a solid phase two-site enzyme
immunoassay based on the sandwich technique, in which two
monoclonal antibodies are directed against separate antigenic
determinants on the leptin molecule. Leptin in the sample
reacts with anti-leptin antibodies bound to microtitration
wells and peroxidase-conjugated anti-leptin antibodies in the
Summary of protocol
The following cross-reactivities have been found:
|CNTF||< 0.05 %|
|Rat Leptin||0.02 %|
|Ovine Leptin||0.003 %|
n.d.= not detected
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