A high quality enzyme immunoassay for the quantification of free human leptin in serum or plasma.
Read the Directions for Use
Available Control set please check in following product:
Control, Obesity A, B, C / Human 10-1241-01
Mercodia Leptin ELISA provides a method for the quantitative determination of human leptin in serum and plasma.
Format: 1 x 96 wells
Samples: Serum, plasma
Sample volume: 25 μL
Assay range: 0.10 - 5.0 ng/mL
Detection limit: ≤ 0.024 ng/mL
Incubation (min): 120+15
The detection limit is ≤ 0.024 ng/mL as determined with the
methodology described in ISO11843-Part 4.
Recovery upon addition is 91 - 109 % (96 %).
Recovery upon dilution is 92 - 114 % (102 %).
Each sample was analyzed in 4 replicates on 24 different
|Sample||Mean Value||Coefficient of variation|
|(μg/L)||within assay %||between assay %||total assay %|
Serum, EDTA , citrate and heparin plasma can be used.
Grossly lipemic, icteric or haemolyzed samples do not interfere
in the assay.
Mercodia Leptin ELISA is a solid phase two-site enzyme
immunoassay based on the sandwich technique, in which two
monoclonal antibodies are directed against separate antigenic
determinants on the leptin molecule. Leptin in the sample
reacts with anti-leptin antibodies bound to microtitration
wells and peroxidase-conjugated anti-leptin antibodies in the
Summary of protocol
|CNTF||< 0.05 %|
|Rat Leptin||0.02 %|
|Ovine Leptin||0.003 %|
n.d.= not detected
Canivell, S, Ruano, E, G (2013)
Gastric Inhibitory Polypeptide Receptor Methylation in Newly Diagnosed, Drug-Naive Patients with Type 2 Diabetes: A Case-Control Study
Anagnostis, P, Selalmatzidou, D, Polyzos, S (2011)
Comparative effects of rosuvastatin and atorvastatin on glucose metabolism and adipokine levels in non-diabetic patients with dyslipidaemia: a prospective randomised open-label study
Spanos, N, Tziomalos, K, Macut, D (2012)
Adipokines, Insulin Resistance and Hyperandrogenemia in Obese Patients with Polycystic Ovary Syndrome: Cross-Sectional Correlations and the Effects of Weight Loss