Higher concentration than expected

Reconstitution volume (for kits with lyophilized calibrators or controls)

The lyophilized calibrators have been reconstituted with a larger volume than stated in the Directions for Use.

Reconstitute with recommended volume of redistilled water. Mix well before using.

Dilution

The samples and/or controls have not been diluted enough.

Always use the volumes recommended for samples, calibrators, controls and enzyme conjugate solution. Check that pipettes are calibrated.