1.0 Mercodia ELISA Technology
Mercodia kits are based strictly on stable, monoclonal antibodies combined with other top quality reagents. This ensures safe long-term production with minimal lot-to-lot variation. By the careful selection of reagents, Mercodia products provide accurate results by first run.
The links underneath will lead you through the principle of the ELISA technology, the difference between sandwich and competitive type of assays, the advantages of monoclonal antibodies and the accuracy of Mercodia´s assays. You will learn more about the hook effect and detection limit and how to calculate your results. And last but not least how you can establish your own controls.
1.1 Principle of Technology
1.2 Polyclonal vs monoclonal antibodies
1.3 Accuracy
1.4 High dose hook effect
1.5 Detection limit
1.6 Calculation of results
1.7 Establishment of control ranges and target values
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